Table 3.
Effect of dietary supplementation of selenium on plasma antioxidant parameters of weanling pigs in response to diquat challenge (n 6)
(Mean values with their standard errors)
| Items | Diquat challenge | sem | P | ||||
|---|---|---|---|---|---|---|---|
| NC | PC | SS | SC | SY | |||
| 0 d (baseline) | |||||||
| SOD (U/ml) | 29·69 | 31·01 | 32·29 | 31·37 | 36·61 | 3·29 | 0·51 |
| GPx (nmol/min per ml) | 303·1b | 299·1b | 301·9b | 391·4a,b | 421·1a | 46·37 | <0·05 |
| TAC (mmol Trolox/ml) | 1·17 | 1·39 | 1·74 | 1·51 | 1·44 | 0·20 | 0·07 |
| MDA (µmol/ml) | 6·89 | 7·61 | 6·73 | 6·45 | 4·86 | 1·26 | 0·43 |
| 6 h PI | |||||||
| SOD (U/ml) | 31·87 | 31·11 | 41·50 | 37·56 | 35·24 | 4·51 | 0·25 |
| GPx (nmol/min per ml) | 350·6a,b | 309·9b | 320·1b | 361·7a,b | 427·0a | 36·90 | <0·05 |
| TAC (mmol Trolox/ml) | 1·30 | 1·31 | 1·76 | 1·47 | 1·49 | 0·18 | 0·08 |
| MDA (µmol/ml) | 5·87 | 8·55 | 6·26 | 4·98 | 6·36 | 1·07 | 0·09 |
| 24 h PI | |||||||
| SOD (U/ml) | 24·93c | 30·76b,c | 36·33a,b | 42·20a | 39·17a | 2·94 | <0·01 |
| GPx (nmol/min per ml) | 417·7 | 305·6 | 372·3 | 368·0 | 398·6 | 44·32 | 0·47 |
| TAC (mmol Trolox/ml) | 1·30 | 1·55 | 1·75 | 1·75 | 1·68 | 0·21 | 0·10 |
| MDA (µmol/ml) | 5·54 | 7·47 | 6·10 | 5·51 | 5·20 | 1·01 | 0·13 |
| 2 d PI | |||||||
| SOD (U/ml) | 28·73b | 34·34a,b | 39·17a | 33·16a,b | 38·55a | 3·19 | <0·05 |
| GPx (nmol/min per ml) | 397·3a | 284·2b | 306·8b | 360·0a,b | 394·8a | 32·74 | <0·05 |
| TAC (mmol Trolox/ml) | 1·34b | 1·30b | 1·66a | 1·68a | 1·46a,b | 0·14 | <0·05 |
| MDA (µmol/ml) | 4·81 | 7·46 | 5·52 | 6·78 | 5·38 | 0·82 | 0·09 |
| 4 d PI | |||||||
| SOD (U/ml) | 24·58b | 28·95b | 41·45a | 34·77a,b | 37·60a | 2·64 | <0·01 |
| GPx (nmol/min per ml) | 398·2 | 307·0 | 367·9 | 335·2 | 401·6 | 56·71 | 0·50 |
| TAC (mmol Trolox/ml) | 1·36 | 1·23 | 1·51 | 1·42 | 1·31 | 0·24 | 0·83 |
| MDA (µmol/ml) | 4·19 | 5·56 | 4·85 | 4·68 | 4·53 | 0·75 | 0·78 |
| 7 d PI | |||||||
| SOD (U/ml) | 27·70b,c | 27·03c | 30·35b,c | 37·66a | 33·57a,b | 2·63 | <0·05 |
| GPx (nmol/min per ml) | 376·1 | 222·4 | 358·9 | 306·1 | 403·3 | 48·08 | 0·09 |
| TAC (mmol Trolox/ml) | 1·41 | 1·40 | 1·59 | 1·31 | 1·37 | 0·15 | 0·70 |
| MDA (µmol/ml) | 4·17 | 4·67 | 5·25 | 4·88 | 4·93 | 0·99 | 0·95 |
NC, negative control; PC, positive control; SS, sodium selenite; SC, soyabean protein-chelated Se; SY, selenised yeast; baseline, before injection; SOD, superoxide dismutase; GPx, glutathione peroxidase; TAC, total antioxidant capacity; MDA, malondialdehyde; PI, post-injection.
a,b,c Mean values within a row with unlike superscript letters were significantly different (P < 0·05).