TABLE 3.
Tm values for TP0037 with or without ligands present
| TP0037 protein |
Tm value (°C)a
for TP0037 with the following additive: |
|||
|---|---|---|---|---|
| No additive | 5 mM NAD+ | 20 mM d-lactate | 20 mM l-lactate | |
| Wild-type | 49.10 ± 0.16 | 53.38 ± 0.09 | 50.12 ± 0.07 | 49.336 ± 0.011 |
| Y101A | 53.48 ± 0.06 | 56.97 ± 0.05 | 53.79 ± 0.06 | 53.48 ± 0.03 |
| R235A | 55.85 ± 0.03 | 59.41 ± 0.05 | 56.207 ± 0.016 | 55.91 ± 0.02 |
| H296A | 48.68 ± 0.07 | 51.69 ± 0.17 | 48.810 ±0.018 | 48.71 ± 0.06 |
a
All results are the means ± standard deviations from three separate Tm determinations.