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. Author manuscript; available in PMC: 2021 Sep 18.
Published in final edited form as: ACS Chem Biol. 2020 Aug 26;15(9):2485–2492. doi: 10.1021/acschembio.0c00478

Figure 6.

Figure 6.

Vesicle budding and collapse in live cells induced by Tat. Cells were incubated with psTopFluor (1.5 μM, green channel) for 15 min on ice, washed with DPBS, treated with TatpHAb (2.5 μM, red channel) for 5 min, and imaged every 4.5 s. (A) Merged image of two HeLa cells (DIC, green, and red channels). (B) Time-lapse confocal microscopic images of the area boxed in red from (A) at 1717–1731 s after initiation of imaging. A single endosome split into two vesicles at 1727 s and one of the vesicles collapsed (marked by a white arrow) while the other remained intact. Scale bars = 1 and 20 μm.