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. 2020 Sep 24;20:463. doi: 10.1186/s12935-020-01505-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — NNT-AS1 served as miR-496 sponge in PCa cells. a Subcellular fractionation assay demonstrated the location of NNT-AS1 in PCa cells. b FISH experiments further proved the distribution of NNT-AS1 in PCa cells. c MS2-RIP assays were conducted in both VCaP and PC3 cells to assess the binding of 9 miRNA candidates toNNT-AS1. d RT-qPCR detected the expression of miR-496 in PCa cells. e ENCORI revealed the binding sites between NNT-AS1 and miR-496. f RNA pull down assay was performed to attest the enrichment of NNT-AS1 in indicated groups. g Luciferase reporter assay further validated the interaction between NNT-AS1 and miR-496 in VCaP and PC3 cells. *p < 0.05, **p < 0.01