Table 2.

Evaluation of the specificity of bCUBE-based qRT-PCR for DENV2 and ZIKV detection

Family	Genus	Species	Strain	BEI No	RNA concentration (ng/µl)	No. of positives for ZIKV assay	No. of positives for DENV2 assay
Flaviviridae	Flavivirus	Japanese encephalitis virus	India	NR-9592	0.1013	0/3	0/3
		West Nile virus	CO 1862	NR-50434	0.10	0/3	0/3
		Yellow fever virus	17D	NR-2869	0.120	0/3	0/3
Togaviridae	Alphavirus	Chikungunya virus	St Martin 2013	NR-50130	0.167	0/3	0/3

Notes: A cross-reactivity panel of frequently co-circulating viruses was used to evaluate the specificity of bCUBE-based qRT-PCR for DENV2 and ZIKV. Arboviral RNA samples were obtained from BEI Resources. RNA concentration (ng/µl) and number of positively detected samples from the ZIKV and DENV2 assay are listed