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Sequences and ELISAs for single‐chain immunoglobulin G (scIgG) binding to Her2 or Her3. Complement determining regions (CDRs) shaded in gray match the template sequence and dashes indicate gaps in the alignment. Amino acids are shaded as follows: Tyr (yellow), Ser (red), Gly (green), and Ala (blue). ELISAs were performed directly with cell culture supernatants from HEK‐293T cells secreting the indicated scIgG. Yields of scIgG or IgG are shown for proteins purified from HEK‐293F cells
