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The pH‐dependence of inhibitor‐induced Tm shifts for Hsp90α (green), Grp94 (red) and Trap1(blue) NTD with 50 μM radicicol, AUY922 and EC144, and 1 mM ADP with 1 mM MgCl2. Additionally, Hsp90α D93N (gray) is shown with 50 μM radicicol. Error bars are the SEM of 3 measurements. Buffer conditions are described in Methods section
