Table 1.
Primers used in this study.
| Primer number/name | 5′ to 3′ sequence |
|---|---|
| 1/pPDR12F | CGAGGTCGACGGTATCGATCTAAACCAAAGATGGATTGTTTACCA |
| 2/pPDR12R | ACCTTTAGACATTTTTTTATTAATAAGAACAATAACA |
| 3/pYGP1F | ATCGAATTCCTGCAGCCCAGCGTGCTATTTTTTAAAAAGGGC |
| 4/pYGP1R | CCTTTAGACATTTTCTATTACTGTATTACTTAACTGACGA |
| 5/pTPO2F | GCCGCCACCGCGGTGGAGCCTATGCAAAAACCCTTCCCC |
| 6/pTPO2R | CCTTTAGACATATTTGTTTTGTGTATTATTTTTGTGA |
| 7/pPDR12-yEGFPF | ATTAATAAAAAAATGTCTAAAGGTGAAGAATTATTCACTGG |
| 8/pPDR12-yEGFPR | GGAATTCGATATCAAGCTTAATATTACCCTGTTATCCCTAGCGG |
| 9/pYGP1-yEGFPF | AGTAATAGAAAATGTCTAAAGGTGAAGAATTATTCACTGG |
| 10/pYGP1-yEGFPR | AGAACTAGTGGATCCCCCATATTACCCTGTTATCCCTAGCGG |
| 11/pTPO2-yEGFPF | ACAAAACAAATATGTCTAAAGGTGAAGAATTATTCACTGG |
| 12/pTPO2-yEGFPR | AGGGAACAAAAGCTGGAGATATTACCCTGTTATCCCTAGCGG |
| 13/crRNAF | CGAGGTCGACGGTATCGAGCTTCTTTGAAAAGATAATGT |
| 14/crRNAR | GGAATTCGATATCAAGCTTAGGCCGCAAATTAAAGCCTTC |
| 15/pRSF | TAAGCTTGATATCGAATTCC |
| 16/pRSR | TCGATACCGTCGACCTCG |
| 17/Cas9F | ATCGAATTCCTGCAGCCCCATAGCTTCAAAATGTTTCTACTCCT |
| 18/Cas9R | AGAACTAGTGGATCCCCCGGCCGCAAATTAAAGCCTTC |
| 19/pWAR1-crRNAF | TAGTGTGTATTGACTGTGATGTTTTAGAGCTAGAAATAGCAAGTTA |
| 20/pWAR1-crRNAR | ATCACAGTCAATACACACTAGATCATTTATCTTTCACTGCG |
| 21/pPDR12F | TTACAAGTTCGTGCATATATAGAAAGAATTCTGTTGTTGTAATTGTCATAACTATTGAGCTCTAAACCAA AGATGGATTGTTTACCA |
| 22/pPDR12R | ATTATCATTATTGATTTCTTTCCCGACGGCAACGCCAGTTATTGCAATCTGCGTGTCCATTTTTTTATTAA TAAGAACAATAACA |
| 23/pWAR1checkF | AACCTGCTGAACCAACAAAACC |
| 24/pWAR1checkR | AACTTTTTGGTCGGTCTTTG |
| 25/4HB-KanMX F | GCCGCCACCGCGGTGGAGTAGGTCTAGAGATCTGTTTAGCTTGC |
| 26/4HB-KanMX R | AGGGAACAAAAGCTGGAGATTAAGGGTTCTCGAGAGCTCG |
| 27/pPDA1 F | GTATTCTGATAAATCTAAAGAGA |
| 28/pPDA1 R | TGGCACAAATGTGGTTTCCT |
| 29/mCherry F | ATGGTTTCTAAGGGTGAAGAAGACA |
| 30/mCherry R | TTACTTGTACAATTCGTCCATACCAC |
| 31/CYC1t F | AAGGCCCCTTTTCCTTTGTC |
| 32/CYC1t R | CGACGATGAGAGTGTAAACTGC |
| 33/UBiC-pTEF1 F | TATTATGTCGACCTCGAGGCACACACCATAG |
| 34/UBiC-CYC1t R | TATTATGCGGCCGCACGATGAGAGTGTAAACTGC |