Table 3.
Yeast strains used in this study.
| Name | Genotype, plasmids | Notes | Origin |
|---|---|---|---|
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Haploid auxotrophic laboratory strain, mating type ‘a’ | Euroscarf |
| BY4742 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | Haploid auxotrophic laboratory strain, mating type ‘α’ | Euroscarf |
| PDR12 | BY4742, pPDR12-GFP-415 | PDR12 promoter regulated GFP expression | This study |
| YGP1 | BY4742, pYGP1-GFP-415 | YGP1 promoter regulated GFP expression | This study |
| TPO2 | BY4742, pTPO2-GFP-415 | TPO2 promoter regulated GFP expression | This study |
| 415.C | BY4741, pRS415, pRS416 | No GFP control strain | This study |
| 425.C | BY4741, pRS425, pRS416 | No GFP control strain | This study |
| GFP.415 | BY4741, pPDR12-GFP-415, pRS416 | Centromeric plasmid biosensor strain | This study |
| GFP.415.U | BY4741, pPDR12-GFP-415, U-416 | Centromeric plasmid biosensor strain with UBiC expression | This study |
| GFP.415.UA | BY4741, pPDR12-GFP-415, UA-416 | Centromeric plasmid biosensor strain with UBiC and ARO4 expression | This study |
| GFP.415.UA.KanMX | BY4741, pPDR12-GFP-415, UA-KanMX-416 | Centromeric plasmid biosensor strain with UBiC, ARO4, and KanMX expression | This study |
| GFP.425 | BY4741, pPDR12-GFP-425, pRS416 | Episomal plasmid biosensor strain | This study |
| +FB.GFP.415 | BY4741, pWAR1::pPDR12, pPDR12-GFP-415, pRS416 | Positive feedback, centromeric plasmid biosensor strain | This study |
| +FB.GFP.415.U | BY4741, pWAR1::pPDR12, pPDR12-GFP-415, U-416 | Positive feedback, centromeric plasmid biosensor strain with UBiC expression | This study |
| +FB.GFP.415.UA | BY4741, pWAR1::pPDR12, pPDR12-GFP-415, UA-416 | Positive feedback, centromeric plasmid biosensor strain with UBiC and ARO4 expression | This study |
| +FB.GFP.mCherry.415 | BY4741, pWAR1::pPDR12, pPDR12-GFP-pPDA1-mCherry-415, pRS416 | Positive feedback, ratiometric centromeric plasmid biosensor strain | This study |
| +FB.GFP.mCherry.415. UA | BY4741, pWAR1::pPDR12, pPDR12-GFP-pPDA1-mCherry-415, UA-416 | Positive feedback, ratiometric centromeric plasmid biosensor strain with UBiC and ARO4 expression | This study |