. Author manuscript; available in PMC: 2020 Sep 24.

Published in final edited form as: Methods Mol Biol. 2012;837:135–148. doi: 10.1007/978-1-61779-504-6_9

Table 1.

Composition of the reaction mixture for the determination of dNTPs (see Note 18)

Reagent	Volume (μl)	Final concentration
1-M Tris-HCl, pH 7.5	0.8	40 mM
0.1-M MgCl₂	2	10 mM
0.5-M DTT	0.2	5 mM
5-μM specific primed oligonucleotide	1	0.25 μM
15-μM radiolabeled deoxynucleotide	1	0.75 μM
Thermo sequenase (32 U/μl)	0.016	0.025 U/μl
Water	10	-
Extract (or standard)	5	1:4
Total volume	20	-