REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-T7 tag	Invitrogen	Cat# PA1-32386; RRID:AB_1960902
Anti-Human IgG	Li-Cor	Cat# 926-32232, RRID:AB_10806644
Anti-Rabbit IgG	Li-Cor	Cat# 926-68071, RRID:AB_10956166
Anti-Glial Fibrillary Associated Protein	Agilent	Cat# Z033429-2
Anti-IgG conjugated to Phycoerythrin	Thermo Scientific	Cat# 12-4998-82, RRID:AB_465926
Bacterial and Virus Strains
BL5403	Novagen, T7 Select Kit	Cat# 70550-3
T7 Bacteriophage	Novagen, T7 Select Kit	Cat# 70550-3
Biological Samples
COVID-19 Patient Sera	This paper	N/A
Pre-Pandemic Control Sera	This paper	N/A
Chemicals, Peptides, and Recombinant Proteins
Blocking Powder	BioRad	Cat# 1706404
Protein A conjugated magnetic beads	Invitrogen	Cat# 10008D
Protein G conjugated magnetic beads	Invitrogen	Cat# 10009D
T4 ligase	New England Bio	Cat# M0202S
Phusion DNA Polymerase	New England Bio	Cat# M0530L
MagPlex Magnetic Beads	Luminex	Cat# MC10012
Coupling Kit	Luminex	Cat# 40-50016
Critical Commercial Assays
T7 Select 10-3b Cloning kit	EMD Millipore	Cat# 70550-3
Ampure XP Beads	Beckman Coulter	Cat# A63881
Deposited Data
VirScan tabulated peptide counts	This paper	https://github.com/UCSF-Wilson-Lab/sars-cov-2_ReScan_VirScan_complete_analysis
ReScan image analysis and hit tables	This paper	https://github.com/UCSF-Wilson-Lab/sars-cov-2_ReScan_VirScan_complete_analysis
Recombinant DNA
Human Coronavirus Synthetic DNA	Twist Bioscience	https://www.twistbioscience.com/
Software and Algorithms
dotblotr	This paper	https://github.com/czbiohub/dotblotr
cd-hit	Li and Godzik, 200637	http://weizhongli-lab.org/cd-hit/
Bowtie2	Langmead and Salzberg38	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
Samtools	Li et al., 200939	http://samtools.sourceforge.net/
Flash	Magoč and Salzberg, 201140	https://ccb.jhu.edu/software/FLASH/
R	Open Source	https://ccb.jhu.edu/software/FLASH/
Bioconductor	Open Source	https://www.bioconductor.org
numpy	Open Source	https://dx.doi.org/10.1109/MCSE.2011.37
skimage	Open Source	https://peerj.com/articles/453
scipy	Open Source	https://www.nature.com/articles/s41592-019-0686-2
pandas	Open Source	https://zenodo.org/record3509134
Matplotlib	Open Source	https://ieeeexplore.ieee.org/document/4160265
openCV	Open Source	https://github.com/skvark/opencv-python
Other
0.2μm filter plates	Arctic White	Cat# AWFP-F20080