| STEP | TEMP | TIME |
|---|---|---|
| Initial Denaturation | 98°C | 30 s |
| 5 Cycles | 98°C | 5 s |
| 70°C | 20 s | |
| 72°C | 15 s | |
| Final Extension | 72°C | 2 minutes |
| Hold | 10°C | - |
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