TABLE 4.

List of downregulated KEGG pathways in CHIR99021-treated HAVICs known to play a role in the pathogenesis of AVS.

KEGG term	KEGG pathway	Observed protein count	FDR	Proteins
hsa04810	Regulation of actin cytoskeleton	31	1.36E-10	ACTN4, ARHGEF1, ARHGEF7, CFL2, CYFIP1, DIAPH1, EGFR, EZR, F2R, ITGA11, ITGA2, ITGA3, ITGA7, ITGA8, ITGAV, ITGB1, ITGB5, MAPK1, MYH10, MYH14, MYH9, MYL9, MYLK, PDGFRA, PDGFRB, PPP1R12A, PPP1R12C, RRAS, RRAS2, VCL, WASL
hsa04151	PI3K-Akt signaling pathway	33	8.06E-07	AKT1, CCND1, CDK4, COL1A1, COL1A2, COL4A1, COL6A1, COL6A2, COL6A3, EGFR, EPHA2, F2R, GNB4, HSP90AA1, HSP90AB1, HSP90B1, ITGA11, ITGA2, ITGA3, ITGA7, ITGA8, ITGAV, ITGB1, ITGB5, LAMC1, MAPK1, NTRK2, PDGFRA, PDGFRB, PRKCA, RHEB, RPS6, YWHAH
hsa05418	Fluid shear stress and atherosclerosis	14	0.0013	AKT1, ASS1, CAV1, GPC1, GSTM2, HSP90AA1, HSP90AB1, HSP90B1, ITGAV, NQO1, PLAT, SQSTM1, SUMO2, TXN
hsa03013	RNA transport	14	0.0054	CYFIP1, EIF2B4, EIF2S2, EIF3A, EIF3B, EIF3D, EIF3G, EIF3H, EIF3I, EIF5, EIF5B, RANGAP1, SUMO2, TPR
hsa04010	MAPK signaling pathway	18	0.0244	AKT1, CACNA2D1, EGFR, EPHA2, FLNA, FLNB, FLNC, HSPA2, HSPB1, MAPK1, MAPKAPK3, NTRK2, PDGFRA, PDGFRB, PRKCA, RRAS, RRAS2, TRADD
hsa04270	Vascular smooth muscle contraction	10	0.0247	ARHGEF1, CALD1, GNAS, MAPK1, MYL9, MYLK, PPP1R12A, PPP1R12C, PRKCA, PRKG1
hsa04210	Apoptosis	10	0.0427	AKT1, CAPN1, CAPN2, CTSD, CTSK, MAPK1, PARP4, SPTAN1, TRADD, TUBA4A
hsa04015	Rap1 signaling pathway	13	0.0441	AKT1, EGFR, EPHA2, F2R, GNAI2, GNAS, ITGB1, MAPK1, PDGFRA, PDGFRB, PRKCA, RRAS, TLN1
hsa04014	Ras signaling pathway	14	0.0454	AKT1, EGFR, EPHA2, EXOC2, GNB4, MAPK1, NTRK2, PDGFRA, PDGFRB, PRKCA, PTPN11, RRAS, RRAS2, TBK1
hsa04630	Jak-STAT signaling pathway	11	0.0454	AKT1, CCND1, EGFR, FHL1, IL13RA2, IL6ST, PDGFRA, PDGFRB, PTPN11, STAT2, STAT6

AVS, aortic valve stenosis. FDR, false discovery rate.