Table 2.

Molecular laboratory techniques, portable biosensing systems and biosensors exploiting Type II Cas effectors.

Name	Cas enzyme	Target		Amplification	Transduction method	LOD	Linear range	Detection time	Sample	Year	Ref
DNA-FISH	dCas9	S.aureus mecA		/	Fluorescence	10 CFU/mL	10-107 CFU/mL	1 h	Genetic DNA or Cell lysate	2017	[56]
CAS-EXPAR	Cas9	ssDNA		EXPAR	Real-Time Fluorescence	0.82 amol		1h	Buffer contains Target	2018	[57]
CRISP-Cas9-SDA-RCA	Cas9(H840A)	E. coli 0157:H7		SDA + RCA	Fluorescence mediate by Metal Organic Framework	4.0 * 101 CFU/ml	1.3*102-6.5*104 CFU/ml	2 h	Spike spring water	2020	[58]
NASBA-CRISP (NASBACC)	Cas9	ika Virus		ASBA	olorimetric	fM		h		2016	[59]
CASLFA	Cas9	ASFV	RPA or PCR		Colorimetric (Lateral flow assay)	150 Copies		40 min	Swine serum	2020	[60]
CRISPR-CHIP	dCas9	DMAS	Amplification free		Field-effector transisotr	1.7 fM		15min	buffer	2019	[61]