(A) The length of pollen tubes growing on pollen germination media with different pH.
(B) The length of wild-type or LAT52::NahG pollen tubes treated with 0 µM or 20 µM SA for 3 h. SA-induced inhibition of pollen tube growth is suppressed by SA hydroxylase NahG.
(C) Pollen tube length of different SA receptor and signaling mutants treated with 0 µM or 20 µM SA. The results show that npr1–2, npr3–1, npr4–3, and npr3–1 npr4–3 responded to SA in the same manner as wild-type.
Tukey’s test was used in (A) and two-way ANOVA with Tukey’s test in (B and C). A, C represent comparison from different genotypes with 0 µM SA treatment, and a, c comparison from different genotypes with 20 µM SA treatment. Error bars show ±SEM; n > 100. **p < 0.01. ND, no significant difference.