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. 2019 Oct 3;41(9):1294–1305. doi: 10.1093/carcin/bgz141

Figure 5.

Figure 5.

CUGBP1 regulates cell growth, colony formation, migration and invasion in breast cancer cells. (A, B) CUGBP1 was silenced by siRNA in MCF7 and T47D cells (A), and overexpressed by plasmid transfection in MDA-MB-231 cells (B). Cell viability was assessed using CCK8 assay. ns denotes not significant. *P < 0.05. **P < 0.01. ***P < 0.001. (C–F) Representative images and the quantitative analysis (D, F) of colony formation after CUGBP1 knockdown in MCF7 and T47D cells (C) and overexpression in MDA-MB-231 cells (E). Cells were seeded at a density of 1000 cells/well in six-well plates and allowed for colonization for 14 days. *P < 0.05. **P < 0.01. (G–J) Representative images of the transwell assay after CUGBP1 knockdown in MCF7 cells and T47D cells (G) and overexpression in MDA-MB-231 cells (I). The corresponding quantitative data were obtained by a spectrophotometer reading of optical density at 590 nm after extraction of the crystal violet staining (H, J). *P < 0.05. **P < 0.01. (K–N) Representative images and quantification of the wound-healing assay after CUGBP1 knockdown in MCF7 cells (K, M) and overexpression in MDA-MB-231 cells (L, N). *P < 0.05. **P < 0.01.