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. 2019 Oct 3;41(9):1294–1305. doi: 10.1093/carcin/bgz141

Figure 6.

Figure 6.

CUGBP1 regulates the expression of the IR and the insulin-signaling pathway. (A) Cell lysate were collected from MCF7 cells transfected with control or CUGBP1 siRNA, with or without 10 nM insulin pretreatment for 20 min. Protein levels associated with insulin-signaling pathway were determined by western blotting. GAPDH was used as an internal control. (B–D) Relative protein expressions of p-IR (B), IR (C) and p-Akt (D) in MCF7 cells were measured by densitometric analyses normalized to GAPDH. Data are means ± SEM. ns denotes not significant. *P < 0.05, **P < 0.01. (E) Cell lysate were collected from MDA-MB-231 cells transfected with pcDNA3.1 vector control or pcDNA3.1-CUGBP1 plasmid, with or without 10 nM insulin pretreatment for 20 min. Protein levels associated with insulin-signaling pathway were determined by western blotting. GAPDH was used as an internal control. (F–H) Relative protein expression of p-IR (F), IR (G) and p-Akt (H) in MDA-MB-231 cells were measured by densitometric analyses normalized to GAPDH. Data are means ± SEM. ns denotes not significant. *P < 0.05, **P < 0.01, ***P < 0.001.