Additional Table 2.
Morphometric parameters of CBS+ cells in the proliferative zones and deep layers of the brain of intact trout (Oncorhynchus mykiss) and 1 week after unilateral eye injury
| Brain areas Cell type | Intact animals | Damaged optic nerve | |||
|---|---|---|---|---|---|
| Cell size (µm) | Optical density (UOD) | Cell type | Cell size (µm) | Optical density (UOD) | |
| Telencephalon | |||||
| Dm | – | Undifferentiated | 7.7±0.2/4.8±0.4 | +++ | |
| Undifferentiated | – | ||||
| Oval | 9.0±0.5/8.0±0.6 | +++ | Oval | 8.7±0.5/5.7±0.6 | +++ |
| 11.1±0.6/7.8±1.2 | ++ | 11.0±0.5/6.1±0.7 | ++ | ||
| Dd | |||||
| Undifferentiated | 4.6±0.9/3.2±0.7 | +++ | Oval | 7.7±0.2/4.8±0.4 | +++/++ |
| Oval | 9.0±0.4/7.5±2.4 | +++ | Oval | 8.7±0.5/5.7±0.6 | +++ |
| ++ | 10.9±0.5/6.1±0.8 | ++ | |||
| Dl | |||||
| Undifferentiated | 6.6±1.2/5.6±1.9 | Undifferentiated | 7.7±0.6/5.3±0.8 | +++ | |
| Oval | 8.8±0.6/6.2±1.4 | +++ | Oval | 8.8±0.5/5.9±0.8 | +++ |
| 11.9±1.8/7.1±1.3 | ++ | 10.7±0.5/6.1±0.7 | ++ | ||
| Dc | Undifferentiated | 3.9±0.5/2.8±0.4 | +++ | ||
| Undifferentiated | 5.4±0.9/3.8±0.8 | +++ | 6.0±0.6/4.2±0.6 | +++ | |
| Oval | 7.7±0.5/5.0±1.3 | +++ | Oval | 7.4±0.2/4.7±0.6 | +++ |
| 9.8±0.2/5.7±1.0 | ++ | 9.5±0.6/4.9±0.6 | ++ | ||
| Vl | 5.8±0.3/3.6±0.6 | +++ | Undifferentiated | 6.3±0.5/4.3±0.5 | +++ |
| Undifferentiated | |||||
| Oval | 9.0±0.8/6.0±1.0 | +++ | Oval | 7.5±0.2/5.0±0.5 | +++ |
| 12.2±1.4/7.6±1.2 | ++ | 9.4±0.8/5.3±1.4 | ++ | ||
| Vm | +++ | Undifferentiated | 5.6±0.3/4.5±0.6 | ++ | |
| Undifferentiated | 5.2±1.2/3.9±0.9 | 6.4±0.2/5.0±0.6 | ++ | ||
| oval | 8.8±0.8/4.9±0.8 | +++ | Oval | 7.7±0.6/5.5±0.9 | ++ |
| ++ | 10.2±1.4/5.7±1.3 | +++ | |||
| Vd | – | – | Undifferentiated | 5.4±0.3/4.0±0.4 | +++ |
| 6.5±0.4/4.5±0.6 | +++ | ||||
| – | – | Oval | 7.3±0.2/4.1±0.8 | ++ | |
| Vv | – | – | Undifferentiated | 5.5±0.3/4.3±0.6 | +++ |
| 6.6±0.3/4.9±0.7 | +++ | ||||
| – | – | – | Oval | 7.7±0.6/5.0±1.2 | +++ |
| 10.4±0.8/5.3±0.8 | +++ | ||||
| Tectum | |||||
| SM | |||||
| Undifferentiated | 4.4±0.1/3.1±0.2 | +++ | Undifferentiated | 5.3±0.3/3.5±0.3 | +++/++ |
| 4.5±0.4/3.0±0.5 | +++ | ||||
| Oval | 7.9±1.3/4.2±0.6 | +++ | Oval | 8.8±0.9/4.7±0.2 | +++ |
| 7.3±0.2/4.0±0.4 | +++ | ||||
| 6.5±0.3/4.0±0.8 | +++ | ||||
| – | – | Radial glia | 8.1±0.9/3.6±0.6 | +++ | |
| 6.4±0.5/3.4±0.6 | +++ | ||||
| SGAP | |||||
| Undifferentiated | 5.2±0.4/4.3±0.5 | ++ | Undifferentiated | 5.6±0.3/4.4±0.6 | +++ |
| 6.5±0.3/5.0±0.4 | +++ | ||||
| – | – | Oval | 10.4±0.3/5.0±1.0 | +++ | |
| 8.7±0.6/5.4±1.3 | +++ | ||||
| 7.5±0.3/5.1±0.6 | +++ | ||||
| Bipolar | 11.7±0.4/5.5±0.4 | +++ | |||
| Pia mater | |||||
| Undifferentiated | 5.6±0.7/4.3±1.1 | +++ | Undifferentiated | 6.9±0.3/4.8±0.5 | +++ |
| Oval | 7.9±0.8/4.5±0.5 | +++ | – | – | |
| SGP | |||||
| Undifferentiated | 4.1±0.3/3.1±0.2 | +++ | Undifferentiated | 6.1±0.4/4.5±0.6 | |
| Oval | 5.4±0.9/3.2±0.5 | +++ | Oval | 8.2±0.5/5.2±0.7 | |
| SGC | |||||
| – | – | Oval | 10.6±0.5/7.0±0.5 | +++ | |
| 9.2±0.3/8.1±0.2 | +++ | ||||
| – | Bipolar | 11.3±0.5/5.1±0.3 | +++ | ||
| Undifferentiated | 6.1±0.3/4.1±0.6 | ++ | Undifferentiated | 6.5±0.5/3.6±0.4 | +++ |
| Cerebellum | |||||
| CCb | |||||
| BMP | |||||
| PC | 26.6±1.4/15.9±3.7 | ++ | PC | 26.5±3.7/14.5±1.7 | ++ |
| 17.7±4.6/11.4±0.1 | +++ | 15.9±2.3/11.8±2.4 | ++/+++ | ||
| EDC | 20.3±6.0/7.9±0.1 | +++ | EDC | 33.0±2.8/11.5±1.0 | +++/++ |
| Glial-like cells | 7.3±1.5/5.9±1.6 | +++ | Glial-like cells | 7.5±1.0/4.4±1.2 | ++/+++ |
| 9.7±0.4/6.5±0.9 | +++ | ||||
| GrL | |||||
| Elongated | 24.5±5.1/10.1±3.2 | ++ | Elongated | 23.0±2.5/13.3±1.8 | +++/++ |
| 14.5±0.2/9.7±0.1 | ++ | 15.0±1.3/9.4±0.5 | +++/++ | ||
| Oval | 10.6±1.5/8.9±1.0 | +++ | Undifferentiated* | 6.6±0.4/4.2±0.3 | +++ |
| ML | |||||
| Oval | 10.1±0.7/6.7±1.3 | +++ | Elongated* | 11.4±1.6/4.7±1.3 | +++ |
| Oval | 8.0±0.6/5.3±1.7 | +++/++ | |||
| Undifferentiated* | 6.3±0.6/4.6±0.8 | +++/++ | |||
| BLP | |||||
| PC | 22.5±0.8/16.9±0.7 | +++/++ | PC | 24.0±0.7/13.9±2.8 | +++/++ |
| 15.2±3.1/10.3±3.8 | +++/++ | 15.3±1.7/8.8±1.4 | +++ | ||
| EDC | – | – | EDC | 39.1±1.6/13.9±2.3 | +++ |
| 25.7±3.1/13.9±2.9 | +++/++ | ||||
| Glial-like cells | 8.2±0.3/6.2±1.0 | +++ | Glial-like cells | 6.7±1.4/5.4±0.6 | +++ |
| ML | |||||
| Superficial cells | 4.8±0.7/2.9±0.3 | +++ | Migrated | 6.9±1.2/4.1±1.3 | – |
| Undifferentiated | 5.2±0.7/3.7±0.7 | +++ | Undifferentiated | 5.3±0.6/3.4±0.5 | +++ |
| GrL | |||||
| Oval | 11.6±3.2/7.3±1.4 | ++/+++ | |||
| Undifferentiated | 5.2±0.9/3.5±0.9 | +++ | Undifferentiated | 5.1±0.3/5.1±0.7 | +++ |
| DLP | |||||
| PC | 25.0±4.5/15.9±3.6 | ++ | PC | 14.9±2.7/8.0±0.6 | ++ |
| 15.4±1.1/12.3±1.4 | ++/+++ | PC+ glial-like cells | 7.9±0.6/4.7±0.8 | +++ | |
| 5.7±0.7/5.4±0.5 | +++ | ||||
| GrL | |||||
| Multipolar | 28.0±4.5/13.7±2.4 | + | Multipolar | 25.0±2.4/13.6±1.8 | +++/++ |
| Heterogenous | 18.4±1.2/10.5±2.9 | ++/+++ | Oval | 16.0±1.2/9.4±0.7 | +++/++ |
| Oval | 11.7±3.0/9.4±3.2 | +++ | Undifferentiated* | 6.4±0.6/4.3±0.4 | +++ |
| ML | |||||
| Oval | 8.1±1.3/4.7±0.9 | +++ | Oval | 8.5±0.5/4.9±0.6 | +++ |
| Undifferentiated | 5.5±0.3/3.7±0.9 | +++ | Superficial cells | 6.8±0.1/5.4±0.2 | +++ |
| 5.6±0.7/3.5±0.7 | +++ | ||||
| DMP | |||||
| PC | 29.2±4.0/15.5±2.8 | ++/+++ | PC | 24.3±3.6/15.6±0.9 | ++ |
| 20.3±2.3/12.7±2.6 | ++ | 17.6±2.2/11.6±2.9 | ++ | ||
| DMZ | |||||
| Undifferentiated | 5.4±1.2/3.9±1.0 | +++ | Undifferentiated | 5.3±1.0/2.7±0.5 | +++ |
| GrL | |||||
| Elongated | 18.7±1.8/9.3±0.7 | ++/+++ | Elongated | 26.5±2.8/14.1±1.8 | ++ |
| Oval | 12.7±2.1/8.8±1.8 | ++/+++ | Oval | 13.5±1.8/9.3±1.7 | ++ |
| ML | |||||
| Undifferentiated | 5.7±0.9/3.3±0.6 | +++ | Oval | 7.5±0.5/4.5±1.2 | +++ |
| Superficial cells | 5.3±1.1/3.3±0.4 | +++ | Undifferentiated | 5.7±0.6/3.3±0.9 | +++ |
| Superficial cells | 4.7±0.3/3.3±0.5 | +++ | |||
| GrL, Caudal part of CCb | |||||
| Heterogenous | Homogeneous | ||||
| Large | 26.0±4.9/13.4±1.6 | ++ | Large | 34.0±2.1/6.5±0.7 | ++ |
| Medial-size | 16.1±1.9/11.4±1.3 | ++ | 27.5±3.5/13.9±1.8 | ++ | |
| Homogenous | Medial-sized | 14.5±2.1/9.4±1.7 | ++ | ||
| Oval | 11.9±4.1/7.5±3.7 | +++ | Oval | 9.3±0.2/7.3±1.0 | +++ |
| Undifferentiated | 5.2±0.4/3.8±0.2 | +++ | Undifferentiated | 5.3±0.4/3.9±0.5 | +++ |
| Granular eminentia | |||||
| Multipolar: | |||||
| Type 1 | 22.0±1.6/11.5±2.0 | +++ | Multipolar | 17.8±1.3/14.8±1.4 | ++ |
| Type 2 | 23.3±1.6/13.6±2.3 | ++ | Bipolar | 24.6±1.3/7.11±1.2 | ++ |
| Type 3 | 15.8±3.6/11.6±3.1 | +++/++ | Oval | 8.9±1.1/6.7±1.1 | +++ |
| Oval | 8.3±0.8/5.7±1.0 | +++ | Undifferentiated | 6.5±1.0/4.0±0.5 | +++/++ |
| Superficial cells | 5.4±0.9/4.1±0.7 | +++ | Superficial cells | 5.4±1.2/4.3±0.3 | +++ |
Data are shown as the mean ± SD. Optical density (OD) in CBS+ cells was categorized by the following scale: high (160–120 UOD, corresponding to +++), moderate (120–80 UOD, corresponding to ++), weak (80–40 UOD, corresponding to +), and low (less than 40 UOD, corresponding to –); the initial OD value was measured on the control mounts. Large and small diameters of the cell body are shown through a slash. Cells were morphologically classified according to a previously developed scheme (Pushchina et al., 2018). *indicates additional cell types that appear in the cerebellum after injury. BLP: Baso-lateral part of cerebellar body; BMP: baso-medial part of cerebellar body; CCb: cerebellar body; Dc: dorso-central region of telencephalon; Dd: dorso-dorsal region of telencephalon; Dl: dorso-lateral region of telencephalon; DLP: dorso-lateral part of cerebellar body; Dm: dorso-medial region of telencephalon; DMP: dorso-medial part of cerebellar body; EDC: euryndroid cells; GrL: granular layer; ML: molecular layer; PC: Purkinje cells; SM: stratum marginale; SGAP: stratum griseum et album periventriculare; SGC: stratum griseum centrale; SGP: stratum griseum periventriculare; UOD: Unites of optical density; Vd: ventro-dorsal region of telencephalon; Vl: ventro-lateral region of telencephalon; Vm: ventro-medial region of telencephalon; Vv: ventro-ventral region of telencephalon.