Table 2. TCR abundance and clonality prognostic value.

	ICO/CLX (n = 95)	ICO/FF (n = 112)	ICO/FFPE (n = 163)	MECC (n = 270)	Combined MSS (n = 575)a
TCR abundance
Mean (range)	0.05 (0–0.18)	0.05 (0–0.16)	0.17 (0.01–0.66)	0.12 (0.003–0.63)	0.10 (0–0.66)
IQR (Q1-Q3)	0.04 (0.03–0.07)	0.05 (0.022–0.07)	0.11 (0.10–0.21)	0.09 (0.06–0.15)	0.10 (0.04–0.14)
< Q1	< 0.03 = 22	< 0.022 = 28	< 0.10 = 56	< 0.06 = 62	149
≥ Q1 HR [95% CI]b	≥ 0.03 = 73 0.25 [0.10–0.63]	≥ 0.022 = 84 0.30 [0.12–0.72]	≥ 0.10 = 107 0.41 [0.18–0.93]	≥ 0.06 = 208 0.56 [0.31–1.00]	426 0.39 [0.26–0.57]
P value	0.003	0.007	0.034	0.042	1.31e-06
Clonality index
Mean (range)	0.06 (0.02–0.18)	0.06 (0.02–0.22)	0.11 (0.04–0.52)	0.18 (0.04–0.74)	0.12 (0.02–0.74)
IQR (Q1–Q3)	0.03 (0.04–0.069)	0.04 (0.04–0.08)	0.06 (0.07–0.13)	0.11 (0.09–0.20)	0.07 (0.06–0.13)
< Q3	< 0.069 = 68	< 0.08 = 84	< 0.13 = 103	< 0.20 = 187	404
≥ Q3 HR [95% CI]b	≥ 0.069 = 26 2.32 [0.90–5.97]	≥ 0.08 = 28 3.32 [1.38–7.94]	≥ 0.13 = 59 0.71 [0.30–1.68]	≥ 0.20 = 68 2.45 [1.39–4.32]	155 2.13 [1.44–3.15]
P value	0.08	0.007	0.43	0.002	0.0002
Undetermined (N, %)	1 (1%)	0	1 (0.6%)	15 (5.6%)	0.0002
TCR continuous (log2)
HR [95% CI]	0.71 [0.57–0.89]	0.68 [0.51–0.91]	0.78 [0.49–1.23]	0.87 [0.69–1.10]	0.85 [0.78–0.93]
P value	0.003	0.010	0.29	0.24	0.002
Clonality continuous (log2)
HR [95% CI]	1.26 [0.67–2.35]	1.56 [0.87–2.80]	1.06 [0.57–1.94]	1.28 [0.95–1.72]	1.16 [1.03–1.31]
P value	0.47	0.13	0.86	0.10	0.016

^aPatients with MSI tumors excluded. Each study uses different cutoffs.

^bHR and 95% CI, adjusted by sex, age, tumor location and stage (Stage IIA, IIB) and MSI status.

CI, confidence interval; CLX, Colonomics study; CRC, colorectal cancer; FF, fresh frozen; FFPE, formalin-fixed paraffin-embedded; HR, hazard ratio; ICO, Catalan Institute of Oncology; IQR, interquartile range; MECC, Molecular Epidemiology of Colorectal Cancer study, MSI, microsatellite instable; Q, quartile; TCR, T-cell receptor.