Table 1. INDEL profiles of sgRNAs when transfecting plasmid DNA or transducing RNPs.
| Guide RNA | Plasmid transfection (n = 1) | RNP (n = 3) | ||||
|---|---|---|---|---|---|---|
| Restoring (Actual %) | Non-restoring (Actual %) | Restoring (Relative %)b | Restoring (Actual %) | Non-restoring (Actual %) | Restoring (Relative %) b | |
| Controla | 0.08 | 0.16 | ND | |||
| Sa-gRNA2 | 19.5 | 24.7 | 44.1 | 20.5±4.9 | 32.9±6.5 | 37.8±1.1 |
| Sp-gRNA1 | 21 | 33 | 38.9 | 19.1±1.1 | 34.5±3.4 | 35.9±3.4 |
| Sp-gRNA2 | 21.4 | 16.4 | 56.6 | 31.8±2.5 | 21.7±1.7 | 59.9±0.8*** |
aControl DNA were from cells transfected with plasmid expressing SaCas9 and sgRNA targeting IL2RG.
bRelative percentage is the percentage of restoring INDELs out of all INDELs observed in that sample. Mean±SEM are listed.
*** indicates p<0.0001 compared with Sa-gRNA2 or Sp-gRNA1 (Tukey’s Multiple Comparison Test following ANOVA).