Table 2. Single guide RNA profiles in myoblasts^a.

	DMD 50 sgRNAs	DMD 53 Sa-gRNA2	DMD 53 Sp-gRNA1			DMD 53 Sp-gRNA2
LVLP-RNPs (ng p24)	400	400	100	200	200	100	200	400
Total Readings	80718	95391	39032	44434	41688	36037	41562	88076
Readings with INDELs	95	109	512	998	1137	870	4987	10294
P (Chi square)	N/A	0.89	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
Total INDEL %	0.12%	0.11%	1.3%	2.24%	2.7%	2.4%	4.8%	11.7%
Restoring (Relative %)b	N/A	N/A	28.5%	37.6%	26.7%	53.8%	52.0%	49.9%

^aIndicated amounts of lentiviral capsid packaged Cas9 ribonucleoprotein (ng p24) were delivered to 1x10⁵ myoblasts isolated from hDMDdel52/mdx mice. The target sequences of human DMD exon 53 amplified from gDNA were analyzed by NGS.

^bSignificantly different between Sp-gRNA1 and Sp-gRNA2 (t-test, p<0.05).