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Generation of glutamyl-pABA from folates and its analysis by LC-MS. (A) Zinc cleavage of folates extracted from E. coli cells to produce glutamyl-pABA. (B) Total ion current chromatogram of partially purified glutamyl-pABA (9.6 min) from E. coli ΔpabA-CT610 cell extracts; (C) the corresponding MS/MS spectrum of glutamyl-pABA showing the [M − H]− molecular ion and several characteristic fragment ions.
