FIG 3.

Confirmation of gene dysregulation in the rne truncation strain. RNA was isolated from B. abortus 2308 or the B. abortus rne truncation strain, and following reverse transcription, quantitative PCR was used to assess the levels of cDNA corresponding to mRNAs from specific genes related to metal homeostasis in B. abortus (bab_rs17810 [encoding a Fur family regulator], ompW, and mntH). 16S rRNA levels were assessed as a control, and all relative gene expression values were normalized to B. abortus 2308. The graphic bars depict the average level of the specified gene for each strain, and the error bars represent the standard error of the mean (SEM).