TABLE 3.
List of strains
|
Strain |
Genotype | Source or referencea |
|---|---|---|
| TH437 | Wild-type strain LT2 | Lab collection |
| TH1184 | F'152-2/nadA56 nadD364::MudJ | |
| TH1898 | pncB::Tn10dCm | |
| TH2117 | sppA::pSLT::MudJ (Δ975 bp into traH through 676 bp into astA) | |
| TH2119 | sppA::pSLT::MudJ (Δ1,019 bp into traH through 438 bp into spy) | |
| TH4875 | zbe-1023::Tn10 (near nadD) | 7 |
| TH22933 | ΔtyrB946::FKF (retains first five and last five amino acids) | |
| TH23051 | ΔaspC881::tetRA (retains first five and last five amino acids) | |
| TH23053 | ΔaspC882 (clean deletion; retains first five and last five amino acids) | |
| TH23095 | ΔtyrB946::FKF ΔaspC882 | |
| TH23393 | ΔaspC881::tetRA tyrB949 (C18T) | |
| TH23394 | ΔaspC881::tetRA PtyrB950 (G-40A) | |
| TH23395 | ΔaspC881::tetRA PtyrB951 (C-66T) | |
| TH23396 | ΔaspC881::tetRA PtyrB952 (G-21A) | |
| TH23806 | F'152-2/nadA56 nadD956::tetRA (inserted after amino acid 105) | |
| TR6630 | sppA::pSLT (156 bp before PSLT026 inserted 1,055 bp into sppA) | |
| TR6631 | pncB255 (Δ62A) aspC883 (Δ−5 to 1,002 bp; A inserted after 1,075 bp) | |
| TT8361 | pSE200 (mucB::lacZ)/AB1157 thy lexB30 | 42 |
| TT8362 | pSE143 (umuC::lacZ)/AB1157 thy lexB30 | 42 |
| TT9993 | sppA::pSLT pncA247::MudA | |
| TT9994 | pncA247::MudA | |
| TT9997 | sppA::pSLT pncA249::MudA | |
| TT9998 | pncA249::MudA | |
| TT10001 | sppA::pSLT pncA251::MudA | |
| TT10002 | pncA251::MudA | |
| TT10003 | sppA::pSLT pncA252::MudA | |
| TT10004 | pncA252::MudA | |
| TT10005 | sppA::pSLT pncA212::MudA | |
| TT10006 | pncA212::MudA | |
| TT10009 | sppA::pSLT pncA229::MudA | |
| TT10010 | pncA229::MudA | |
| TT10024 | pSE200(mucB::lacZ) | |
| TT10025 | pSE143(umuC::lacZ) | |
| TT10026 | pSE200(mucB::lacZ)/sppA::pSLT | |
| TT10027 | pSE143(umuC::lacZ)/sppA::pSLT | |
| TT10044 | pnc-254::Tn10 (near sppA) | |
| TT10281 | zcg-1819::Tn10 (linked to pncA) | |
| TGL553 | sppA::pSLT(ΔrepC-repA::FCF) (ΔrepC to 1 bp after stop of repA) | |
| TGL555 | sppA::pSLT(ΔrepC-repA2::FCF) (ΔrepC through −1 of repA2) | |
| TGL556 | sppA::pSLT(ΔtraH-PSLT026::FCF) (Δ975 bp of traH to pSLT end) | |
| TGL557 | sppA::pSLT ΔxthA::FCF (deleted all but last bp of xthA) | |
| TGL584 | sppA::pSLT(ΔrepA2-1::FKF) (deleted −1 through stop) | |
| TGL585 | sppA::pSLT(ΔrepA2-2::FKF) (deleted −1 through 855 bp after repA2) | |
| TGL586 | sppA::pSLT(ΔrepA2-pefB::FKF) (deleted −1 to 1,974 bp after repA2) | |
| TGL587 | sppA::pSLT(ΔrepCA-PSLT007::FCF) (ΔrepC to 1,150 bp after repA) | |
| TGL596 | sppA::pSLT(ΔrepC-repA::FCF ΔrepA2-1::FKF) | |
| TGL601 | sppA::pSLT(ΔrepCA-PSLT007::FCF ΔrepA2-pefB::FKF) |
a
Unless noted otherwise, all strains were constructed during the course of this work.