TABLE 2.
E. coli strains and plasmids used for genetic analyses and constructions in this study
| E. coli strain or plasmid | Relevant genotype description | Identifier | Reference, source, or construction |
|---|---|---|---|
| Strains | |||
| CFT073 Strr | Spontaneous streptomycin-resistant mutant of CFT073 | CFT073 | 7 |
| CFT073 | Original clinical isolate | CFT073WT | 45 |
| MG1655 | LAM-rph-1 | MG1655 | 46 |
| BL21(λDE3) | F− ompT gal dcm lon hsdSB(rB− mB−) λ(DE3[lacI lacUV5-T7 gene 1 ind-1 sam7 nin5]) | BL21(λDE3) | EMD Millipore, USA |
| ED0052 | CFT073 Strr ΔlysP::parE-kan | CFT073 ΔlysP | CFT073; λred |
| ED0070 | CFT073 Strr ΔargP::cat | CFT073 ΔargP | CFT073; λred |
| ED0169 | CFT073 ΔlysA::cat | CFT073 ΔlysA | CFT073WT; λred |
| JJ1886 | JJ1886 | 47 | |
| ED0118 | JJ1886 ΔmppA::cat | JJ1886 ΔmppA | JJ1886; λred |
| ED0131 | JJ1886 ΔoppABCDF::cat | JJ1886 ΔoppABCDF | JJ1886; λred |
| Plasmids | |||
| pKD46 | amp | 43 | |
| pKD3 | cat | 43 | |
| pKD267 | kan | B. Wannera; 48 | |
| pKD119 | tet | 43 | |
| pBAD24 | amp (expression vector) | 49 | |
| pLysP | amp Para::lysP | pLysP | This study |
| pArgP | amp Para::argP | pArgP | This study |
| pet-AmiD | kan (ΔN17)amiD-His6 | This study | |
a
J. Teramoto, K. A. Datsenko, and B. L. Wanner, unpublished construction.