Figure 4.

(a) RT-PCR analysis of PI3 K RNA extracted from DU-145 cell clone lysates. Values are normalized to β-actin and are expressed relative to those of control (Scramble shRNA and Control), which are arbitrarily settled to 1. Each value is the mean ± SD from n = 3 independent experiments, while y-axis represents the ratio between PI3 K and actin genes. n.s indicates nonstatistical significance.

Detection of protein expression via western blotting. Western Blot analysis of (b) PI-3 K and (c) p85PI3 K protein expression in DU-145 cell clone lysates. Statistical data of (d) PI3 K and (e) p85PI3 K protein expression levels in DU-145 cell clone lysates. Each value is the mean ± SD from n = 4 experiments. Data are normalized using tubulin as immunoblot loading control. n.s. indicates nonstatistical significance, *(p ≤ 0.05), **(p ≤ 0.01) indicates statistical significance.