Skip to main content
. 2020 Jan 10;21(4):379–387. doi: 10.1080/15384047.2019.1702405

Figure 2.

Figure 2.

LncRNA FTX can interact with miR-320a. (a) MiR-320a was selected form 23 miRNAs that are combinative with FTX. (b) Subcellular fractionation assay and FISH indicated FTX was primarily located in cytoplasm. (c) Potential binding sites of FTX with miR-320a seed sequence on Starbase. (d) Dual-luciferase report proved the interaction between FTX and miR-320a. FTX WT referred to wild-type in FTX sequence to interact sequences of miR-320a pmirGLO luciferase reporter vector. FTX MUT was constructed using mutant binding sites. (e) RNA pull-down confirmed the physical interaction between FTX and miR-320a. (f-g) Relative expression of FTX in OS tissues and cells was observed by qRT-PCR. (h-j) CCK8, EdU and colony formation were used to inspect cell proliferation among shNC, shFTX and shFTX+ miR-320a inhibitor. (k-l) TUNEL and flow cytometry were used to examine cell apoptosis. (m-n) Wound healing and Transwell were used to measure cell migration. (o) Western blot was used to measure proteins expression level. *P < .05, **P < .01.