Skip to main content
. 2020 May 7;21(7):629–636. doi: 10.1080/15384047.2020.1750297

Figure 1.

Figure 1.

Expression of SA-GFP in breast cancer cells does not affect proliferation in vitro. (a, b) Representative histograms of IFNAR1 cell surface levels measured by flow cytometry on GFP and SA-GFP E0771 (a) and AT-3 cells (b). (c) Immunoblot analysis of activated (pSTAT1, pSTAT3) and total Stat1 and Stat3 in E0771 cells line expressing GFP or SA-GFP. Levels of GAPDH were used as a loading control. (d) qPCR analysis of the ISGs mRNA levels in E0771 cells expressing GFP or SA-GFP. The data are shown as mean ± SEM (n = 3). (e, f) Cell proliferation assay of GFP and SA-GFP E0771 (e) and AT-3 (f) cells. Cells (mean±SEM, n = 3) were counted for 3 days after seeding.