Figure 3.

Representative immunohistochemistry images from the intratumoral region of two cases with colorectal liver metastasis. Sections were stained to detect the total amount of T-cells (CD3+) and cytotoxic T-cells (CD8+). On serial slides, corresponding hotspots were selected to quantify T-cells for each case, shown by the black rectangles. (Images were acquired at 4x magnification and the black line represents 0.2 mm).

Case 1 (short-interval NACT subgroup): (a) total number of T-cells. (b) cytotoxic T-cells. Case 2 (no NACT subgroup): (c) total number of T-cells. (d) cytotoxic T-cells