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. 2020 Sep 14;14(9):e0008608. doi: 10.1371/journal.pntd.0008608

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© 2020 Poveda et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — A) Fluorescence microscopy images corresponding to BALB/c and Slamf1^-/- macrophages incubated for 1 h with parasites of the Y strain. The nucleus of macrophages appears stained in blue (Dapi). The arrows point to fluorescent parasites labelled with Mito Tracker Orange attached to macrophages from BALB/c and Slamf1^-/- mice. A representative experiment of two is shown. B) Percentage of peritoneal macrophages from BALB/c and Slamf1^-/- mice interacting with T. cruzi after 1 h. C) Number of trypomastigotes attached to 100 peritoneal macrophages from BALB/c and Slamf1^-/-mice after 1 h. In B and C the mean ± SEM corresponds to 14 fields of each condition performed in duplicate. D) Quantification of the parasite load in macrophages from BALB/c and Slamf1^-/- mice incubated with the Y strain during 1h. The statistical significance of the differences between both strains of mice is indicated: *** p <0.001, ** p <0.01.