FIGURE 2.

Longitudinal analysis of the development of donor DR15–restricted ColV autoimmunity and its regulation. Patient L86 (HLA−DR1,11) received a lung transplant from a HLA 1,15 donor. Note the strong anti−ColV response at year 7, including a strong donor DR15–restricted response to p1049, as well as a DR1−restricted response to p629 as published previously (7). Tv−DTH responses in the same patient were reanalyzed at 14 y post-transplant. The recipient now had low responses to ColV and p1439 and no response to p1049. But neutralization of TGF−β and IL−35 led to an increase in responses to ColV and p1439 and uncovered a response to the DR15−restricted p1049 but not to the self (DR1)−restricted p629. PBMC, peripheral blood mononuclear cells; TGF−β, transforming growth factor−β.