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. 2020 Sep 24;9:e54573. doi: 10.7554/eLife.54573

Figure 3. Nematostella PGCs migrate aborally to the gonad rudiments during the juvenile stage.

(A–A’) The majority of young primary polyps (≤10 dpf) exhibit two PGC clusters (Vas2+, red) in close proximity to the pharynx. (B–B’) In more mature primary polyps (>10 dpf), some PGCs elongate and localize between the mesenteries. (C–C’) Following feeding, putative PGCs spread aborally into the gonad rudiments. (D–D’) A juvenile polyp viewed 90 degrees from the orientation of C, showing aborally migrating PGCs in non-primary mesenteries. Scale bar = 10 µm in A’ and B’; 20 µm in C’ and D’.

Figure 3.

Figure 3—figure supplement 1. PGCs are proliferative in juvenile polyps.

Figure 3—figure supplement 1.

(A) A representative six tentacle-stage juvenile stained with anti-Vas2 (red), EdU (green), anti-Phospho-Histone H3 (pH3, yellow) and nuclei (blue). (B–D) Enlarged view of the boxed area in A. Proliferative PGCs accumulate EdU (arrowheads) or pH3 (arrow) in the nuclei. (C and D) Selected single focal planes from B. Scale bar = 100 µm in A; 20 µm in B. B–D are at the same scale.