Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Nematostella vectensis) | vas1 | GenBank | AY730696 | |
| Gene (Nematostella vectensis) | vas2 | GenBank | AY730697 | |
| Gene (Nematostella vectensis) | piwi1 | GenBank | MF683122 | |
| Gene (Nematostella vectensis) | piwi2 | GenBank | MF683123 | |
| Gene (Nematostella vectensis) | tudor | GenBank | XM_032376221 | |
| Gene (Nematostella vectensis) | twist | GenBank | AY286509 | |
| Gene (Nematostella vectensis) | hh1 | GenBank | EU162651 | |
| Gene (Nematostella vectensis) | ptc | GenBank | EU162650 | |
| Gene (Nematostella vectensis) | gli | GenBank | EU162649 | |
| Gene (Nematostella vectensis) | Anthox6a | GenBank | GQ240845 | |
| Gene (Nematostella vectensis) | gbx | GenBank | DQ500757 | |
| Genetic reagent (Nematostella vectensis) | hh11 | This paper | See Figure 9—figure supplement 1 | |
| Genetic reagent (Nematostella vectensis) | hh12 | This paper | See Figure 9—figure supplement 1 | |
| Genetic reagent (Nematostella vectensis) | hh13 | This paper | See Figure 9—figure supplement 1 | |
| Genetic reagent (Nematostella vectensis) | ptc1 | This paper | See Figure 10—figure supplement 1 | |
| Genetic reagent (Nematostella vectensis) | ptc2 | This paper | See Figure 10—figure supplement 1 | |
| Genetic reagent (Nematostella vectensis) | ptc3 | This paper | See Figure 10—figure supplement 1 | |
| Genetic reagent (Nematostella vectensis) | ptc4 | This paper | See Figure 10—figure supplement 1 | |
| Peptide, recombinant protein | vas2 | This paper | MCFKCQQTGHFARECPNESAAGENGDRPKPVTYVPPTPTEDEEEMFRSTIQQGINFEKYDQIEVLVSGNNPVRHINSFEEANLYEAFLNNVRKAQYKKPTPHHHHHH | |
| Antibody | anti-vas2 (Rabbit polyclonal) | This paper | IF (1:1000), stock: 0.4 mg/mL | |
| Antibody | anti-α-Tubulin (mouse monoclonal) | Sigma-Aldrich | T9026 | IF (1:1000) |
| Antibody | anti-Phospho-Histone H3 (mouse monoclonal) | Sigma-Aldrich | 05–806 | IF (1:1000) |
| Antibody | anti-DIG-POD Fab fragments (Sheep polyclonal) | Sigma-Aldrich | 11207733910 | FISH (1:1000) |
| Antibody | anti-Fluorescein-POD Fab fragments (Sheep polyclonal) | Sigma-Aldrich | 11426346910 | FISH (1:1000) |
| Chemical compound, drug | GDC-0449 | Cayman Chemical | 13613 | 25 µM |
| Chemical compound, drug | Cyclopamine | Cayman Chemical | 11321 | 5 µM |
| Chemical compound, drug | Benzyl alcohol | Sigma-Aldrich | 305197 | |
| Chemical compound, drug | Benzyl benzoate | Sigma-Aldrich | B6630 | |
| Commercial assay or kit | Click-iT EdU Kit | Thermo Fisher Scientific | C10339 | |
| Commercial assay or kit | TSA Plus Cyanine 3 System | PerkinElmer | NEL744001KT | |
| Commercial assay or kit | ImProm-II Reverse Transcription System | Promega | A3800 | |
| Commercial assay or kit | T7 RNA polymerase Kit | Promega | P2077 | |
| Commercial assay or kit | AmpliScribe T7-Flash Transcription Kit | Lucigen | ASF3507 | |
| Software, algorithm | ImageJ | ImageJ (http://imagej.nih.gov/ij/) | RRID:SCR_003070 | |
| Software, algorithm | Imaris | Bitplane | RRID:SCR_007370 | 8.3 |
| Software, algorithm | BoxPlotR | BoxPlotR.shiny (https://github.com/VizWizard/BoxPlotR.shiny/blob/master/README.md) | RRID:SCR_015629 | |
| Other | Hoechst-34580 stain | Sigma-Aldrich | 63493 | 1 µg/mL |
| Other | SYBR Green I stain | Thermo Fisher Scientific | S7567 | 1:5000 |
| Other | Phalloidin stain | Thermo Fisher Scientific | A12379 | 1:200 |
| Other | DIG RNA labeling mix | Sigma-Aldrich | 11277073910 | |
| Other | Fluorescein RNA labeling mix | Sigma-Aldrich | 11685619910 | |
| Other | Cas9 protein with NLS | PNA Bio | CP02 | 500 ng/µL |