Table 1. Primer sets for cloning probe templates into pPR-T4P (gene-specific regions are underlined).

Target	Sequence		Probe size (bp)
piwi1	Forward	CATTACCATCCCGCGAGCCTACAACCAGGAGAG	1327
	Reverse	CCAATTCTACCCGCGTTGTGTTGATGCCCATAG
piwi2	Forward	CATTACCATCCCGTGGGCGGTACTTCTACAACC	1367
	Reverse	CCAATTCTACCCGTGCCCTTGATAAGGAGCATC
vas2	Forward	CATTACCATCCCGTGAAGGGTCTCCAATTCCTG	1530
	Reverse	CCAATTCTACCCGTGTGCAGATTACAGCCAAGG
tudor	Forward	CATTACCATCCCGGAACCTACTTGCTTCCGCAG	1450
	Reverse	CCAATTCTACCCGACGACTCGGTGTTCCCATAG
twist	Forward	CATTACCATCCCGAAATCTCGGTGTCGGTCTTG	1020
	Reverse	CCAATTCTACCCGTATCGCAGCTTTGCTTTCAG
hh1	Forward	CATTACCATCCCGTTTCATTGGGAGCTAGTGGG	1327
	Reverse	CCAATTCTACCCGAAAGCGTGAATTGGGTCTTG
ptc	Forward	CATTACCATCCCGGATGTGCGTGTGTGGGATAG	1455
	Reverse	CCAATTCTACCCGACCGCGAGGTAATTGAACAC