Table 3. gRNA sequence for CRISPR/Cas9 mutagenesis (PAM sequences are underlined).
Target | gRNA name | Sequence |
---|---|---|
hh1 | hh1-gRNAa | GGGAGCTAGTGGGAGACCACAGG |
hh1 | hh1-gRNAb | GCTCATGAGGCGATCGGCACCGG |
ptc | ptc-gRNAa | GGAGGGGTTTGGAGCATCACAGG |
ptc | ptc-gRNAb | AGAGGTGAAGGCCAGGACAGTGG |
gli | gli-gRNAa | GCGGCATGACCAGGAGGAGGTGG |
gli | gli-gRNAb | AGTGAGGTGGCTGTGGATGGTGG |