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. 2020 Sep 24;9:e54573. doi: 10.7554/eLife.54573

Table 3. gRNA sequence for CRISPR/Cas9 mutagenesis (PAM sequences are underlined).

Target gRNA name Sequence
hh1 hh1-gRNAa GGGAGCTAGTGGGAGACCACAGG
hh1 hh1-gRNAb GCTCATGAGGCGATCGGCACCGG
ptc ptc-gRNAa GGAGGGGTTTGGAGCATCACAGG
ptc ptc-gRNAb AGAGGTGAAGGCCAGGACAGTGG
gli gli-gRNAa GCGGCATGACCAGGAGGAGGTGG
gli gli-gRNAb AGTGAGGTGGCTGTGGATGGTGG