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. Author manuscript; available in PMC: 2020 Sep 24.
Published in final edited form as: Neurobiol Dis. 2020 Feb 4;138:104785. doi: 10.1016/j.nbd.2020.104785

Figure 1. Deletion of Psen1 in N2A cells.

Figure 1.

(A) Design for CRISPR/Cas9-mediated knock-out of Psen1 and PCR screening assay using F1 and R1 primers. (B) Functional validation of single and dual gRNA activity for Psen1 targeting. (C) PCR screening of genomic DNA from Psen1 knock-out clones. Black arrows point to the PCR band from wild type or unedited clone. Red arrows point to the bands produced by CRISPR/Cas9-mediated editing. (D) Genomic DNA sequencing results of the N2A-PS1KO-8 clone for two alleles as assessed by PCR in (C). Nucleotides in bold belong to the coding exon. Start codon nucleotides are labeled in red.