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. Author manuscript; available in PMC: 2020 Sep 24.
Published in final edited form as: Neurobiol Dis. 2020 Feb 4;138:104785. doi: 10.1016/j.nbd.2020.104785

Figure 4. Rescue of double Psen1/Psen2 knock-out cells with exogenous PSEN2 expression.

Figure 4.

(A) Analysis of γ-secretase subunits and APP processing by western blotting in wild type (2) and double knock-out (8/71) cells rescued with wild type human presenilin 2 (PS2-WT). Quantification of western blotting data is in Supplementary Figure S1B. (B) Quantification of Aβ40, Aβ42, Aβ42/Aβ40 ratio by ELISA, and (C) total Aβ by western blotting based on (A) versus N2A-WT-2 cells. Values shown are mean ± SD of n = 3 independent experiments with one (C) or two (B) technical replicates each. ** P < 0.01, *** P < 0.001, one-way ANOVA with Dunnett’s post-hoc test against the control condition (2).