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. Author manuscript; available in PMC: 2020 Sep 24.
Published in final edited form as: Neurobiol Dis. 2020 Feb 4;138:104785. doi: 10.1016/j.nbd.2020.104785

Figure 5. The effect of PSEN1 coding mutations associated with AD clinical diagnosis on γ-secretase complex and Aβ production.

Figure 5.

(A) Analysis of γ-secretase subunits, APP and Aβ protein levels in cell lysates and conditioned medium from N2A-PS1/2KO-8/71 line transfected with wild type and mutant PSEN1. Quantification of western blotting data is in Supplementary Figure S2A. (B) Changes in Aβ40, Aβ42, and Aβ42/Aβ40 ratio caused by mutations in PSEN1 compared to wild type PSEN1 measured by ELISA. (C) Quantification of total Aβ (C) based on (A). Values shown are mean ± SD of n = 3 independent experiments with one (C) or two (B) technical replicates each. * P < 0.05, ** P < 0.01, *** P < 0.001, one-way ANOVA with Dunnett’s post-hoc test against control condition (8/71 + PS1-WT).