Figure 5.

Changes in the growth recovery upon expression of different Pub1 variants. (A) Scheme of the different steps followed to prepare the cell samples before the growth curve analysis. The pre-inoculum was in glucose media. Then the cells were incubated in inducing conditions during different times (0, 8, 16 and 24 h) before changing again to the glucose media (inhibition conditions) to stop Pub1 expression and perform the growth characterization. (B) Growth curves after 24 h growing in no-inducing conditions as a fitness control. (C) Growth curves where monitored for 3 days after stopping PUB1 expression (see Supplementary Table S3). The graph exhibits growth curves after 24 h of pre-induction. For other pre-induction times see Supplementary Table S3. (D) Bar plots indicating the doubling time once Pub1 expression is stopped and after different pre-inducing times. For lag time and saturation limit see Supplementary Table S3. Bar high corresponds to arithmetic mean of three independent biological replicates and error bars indicate standard deviations. (E) Linear correlation between the propensity to phase separate calculated by catGRANULE and the lag time measured after 24 h of induction.