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. 2020 Aug 18;48(17):9959–9968. doi: 10.1093/nar/gkaa690

Figure 5.

Figure 5.

Structure comparison of type I-F Acr proteins in complex with P. aeruginosa Cas8f. Complex structure of Cas8f in blue with (A) AcrIF7 in orange (this study), (B) AcrIF2 in red (PDB code 6B47), (C) AcrIF6 in purple (PDB code 6VQX), and (D) AcrIF10 in pink (PDB code 6B48), in a cartoon representation. The structure of the AcrIF7:Cas8f complex was modeled based on the Csy complex structure and mutagenic studies, otherwise complex structures were determined by cryo-EM. The key residues for the interaction are annotated in the AcrIF7:Cas8f structure. All structures are shown in the same perspective. Only the N-terminal region of Cas8f (residues 1–267) responsible for the interaction is shown for visual clarity. Lys28 and Lys247 of P. aeruginosa Cas8f are equivalent to Lys29 and Lys248 of X. albilineans Cas8f, respectively.