Figure 7.

Inhibition of U-insertion RNA-editing by UTP-analogs. (A) Tested UTP-analogs are listed next to a ball-and-stick representation of UMP to positionally categorize the different compounds as CH-edge-, Watson/Crick edge-, phosphate- or ribose-interfering. (B) Z-score representation of the FIDE-assay results for the reaction input, all reaction intermediates and partially edited products as well as the fully edited product and reaction by-products (Z = x – μ/∂ with x = score, μ = mean, ∂ = standard deviation). A reference chart of the different RNA-species is given in Supplementary Figure S2. The heat-map block at the bottom summarizes the incorporation of UTP-analogs in the presence of Mn²⁺ instead of Mg²⁺.