Skip to main content
. 2020 Sep 1;48(17):9840–9858. doi: 10.1093/nar/gkaa715

Figure 2.

Figure 2.

Pre-mRNA increase induced by gapmer ASOs is translation dependent. (A) qRT-PCR quantification of NCL mRNA and pre-mRNA levels in HeLa cells transfected for 4 h with three different ASOs targeting 3′ UTR of NCL mRNA. (B, C) qRT-PCR quantification of NCL pre-mRNA (panel B) and mRNA (Panel C) levels in HeLa cells transfected with 25 nM ASO110074 for 3 h, followed by treatment with either ethanol (EtOH) or 100 μg/ml CHX for an additional 2 h. (D, E) qRT-PCR quantification of NCL pre-mRNA (Panel D) or mRNA (panel E) levels in HeLa cells transfected with 25 nM ASO110074 or ASO110080 for 3 h, followed by treatment with either ethanol (EtOH) or 40 μg/ml puromycin (PUR) for 2 h. (F) qRT-PCR quantification of NCL mRNA and pre-mRNA levels in HeLa cells transfected for 3 h with 25 nM ASO110093, followed by treatment with ethanol or 40 μg/ml puromycin for 2 h. (G) qRT-PCR quantification of NCL mRNA and pre-mRNA levels in HeLa cells transfected for 3 h with 25 nM ASO110108, followed by treatment with either ethanol or 40 μg/ml puromycin for 2 h. The error bars in each panel are standard deviations from three independent experiments. P values were calculated based on F-test using Prism.