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. 2020 Sep 1;48(17):9840–9858. doi: 10.1093/nar/gkaa715

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Pre-mRNA level was not increased by ASOs that do not support RNase H1 cleavage. (A) ASOs that contain different modifications in the gap region that inactivate RNase H1 cleavage. The sequence and modifications of the ASOs are shown. e, 2′MOE; s, phosphorothioate; mC, 5-mC. The ‘m’ in XL761 represents 2′-OMe. (B) qRT-PCR quantification of NCL mRNA (upper panel) and pre-mRNA (lower panel) levels in HeLa cells transfected with different ASOs for 4 h. (C) qRT-PCR quantification of NCL mRNA (upper panel) and pre-mRNA (lower panel) levels in HeLa cells transfected for 4 h with either the parental gapmer ASO or two ASOs that are uniformly modified with 2′MOE. (D) qRT-PCR quantification of NCL mRNA (upper panel) and pre-mRNA (lower panel) levels in HeLa cells transfected with PS-MOE or PO-MOE gapmer ASOs for 4 h. The error bars in each panel are standard deviations from three independent experiments. P values were calculated based on F-test using Prism. NS, not significant.