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. 2020 Sep 1;48(17):9840–9858. doi: 10.1093/nar/gkaa715

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Reduction of UPF3A attenuated pre-mRNA increase induced by gapmer ASOs. (A) qRT-PCR quantification of UPF3A mRNA in HeLa cells transfected for 48 h with 5 nM control luciferase siRNA or UPF3A specific siRNA (ID10476). (B, C) qRT-PCR quantification of NCL mRNA (left panels) and pre-mRNA (right panels) levels in control cells (Luci) or UPF3A-reduced HeLa cells (UPF3A-siRNA) that were subsequently transfected with ASO110074 (Panel B) or ASO110093 (Panel C) for 4 h. (D) Western analysis for WDR5 protein in control luci-siRNA or WDR5 siRNA treated HeLa cells. GAPDH was probed and served as a control for loading. (E, F) qRT-PCR quantification of NCL mRNA (left panels) and pre-mRNA (right panels) levels in control cells (Luci) or WDR5-reduced HeLa cells (WDR5 siRNA) that were subsequently transfected with ASO110074 (Panel E) or ASO110093 (Panel F) for 4 h. The error bars in each panel are standard deviations from three independent experiments. P values were calculated based on F-test using Prism. NS, not significant.