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. 2020 Sep 1;48(17):9840–9858. doi: 10.1093/nar/gkaa715

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — Certain gapmer ASOs targeting SOD1 mRNA can increase SOD1 pre-mRNA levels. (A) qRT-PCR quantification of the levels of SOD1 mRNA and pre-mRNA in HeLa cells transfected with different ASOs at 25 nM for 4 h. ASOs targeting 5′ UTR, CDS, and 3′ UTR are indicated. Two ASOs showing mRNA reduction and pre-mRNA increase are marked with red arrowheads. (B, C) qRT-PCR quantification of SOD1 mRNA (left panels) and pre-mRNA (right panels) levels in cells transfected for 48 h with siRNAs specific to luciferase (luci), UPF2, UPR3A and RNase H1 that were subsequently transfected with ASO150457 (Panel B) or ASO150461 (Panel C) for an additional 4 h. The error bars in each panel are standard deviations from three independent experiments. P values were calculated based on F-test using Prism.