Table 1.
The minimum set of gRNA classes involved in the editing of the T. lewisi A6 mRNA
| mRNA 5′ | Length of the comple-mentarity | RPM* | gRNA classes of A6 | Minicircle no. |
|---|---|---|---|---|
| −4 | 47 | 880 | ATATAA ACGTAACCAGAACGGATTACAGAGAGAAAATAAGAATGTGATAAT 2 t 4 | TLminic2 |
| 22 | 52 | 11411 | ATATAG ATCACACACGGTAATATAGTTATGATAATGAATGTAGCTGAGATGGATTTt 5 | TLminic33 |
| 58 | 45 | 361 | ATATAA AATTAAAATACACAATAGAACATAACCTAGATTACATGTGGTAGT 4 t 3 | TLminic56 |
| 90 | 52 | 2804 | ATGTGA TATATAAACAAAACAACAAAGAATCGAGAGTTAACGTTGAATGATTAAAGTGTGt 4 | TLmcontig13 |
| 126 | 51 | 309 | AATAAA TAAACATAAACTAACAGATGGGTATAGGTTAGATAGATAAAATGGTGATt 6 | TLmcontig3 |
| 162 | 44 | 13263 | ATTAAA ATTAAACAACATAGATCACGGTGATGAAAATGGATGTAGATCT 3 t 8 | TLmcontig7 |
| 189 | 44 | 10549 | GATATA TACAATAATACAAGATCGGGCTGTATGAATTAGGTGATGTGAAAT 3 t 5 | TLminic17 |
| 213 | 46 | 716 | ATATAA CAAATACAGATTCAGGTAGGTGGTATAGTGGTATAGAATCGGTGGAAT 3 t 4 | TLminic1 |
| 244 | 52 | 3756 | AGAGAT GGCATGTAATACAAAAAGATGATACAAAATAATAGATATGAATTTATt 9 | TLmcontig1 |
| 245 | 50 | 1399 | ATTAAA ATGACATATGATACAAAGAGATAGTATGGGATAACAGATATGAATTt 7 | TLminic39 |
| 283 | 42 | 104 | GTATTA AAATTCAACAAGAAACAAAACAGAAATAGAGGTGGTGTATATAT 2 t 6 | TLminic16 |
| 314 | 50 | 6506 | ATATAA AACTATCACTAACTAATGGGCTGTTAGATAGAGAGAAATTTAACGTGTt 6 | TLmcontig25 |
| 317 | 49 | 2933 | ATATTT TTATAAAACTATCATCAACTGGCAGATTATTAGATAGAGGAGAATTTAt 7 | TLminic41 |
| 351 | 45 | 15337 | ATAGAT AAACACAACAAAGAACGGATAGAAGAAATATCTATGAGATTATTAt 9 | TLmcontig23 |
| 382 | 47 | 15945 | ATACAT AACGGCACAATAAAGAAGAACGTTCTAGAGAATATAATAAGAAATt 8 | TLmcontig17 |
| 413 | 46 | 121 | GTAAAT ATTACAAAACAAACGTGAAGATGTTGATAAGCAATGATATGATAAAT 3 t 5 | TLminic34 |
| 455 | 44 | 198 | ATATAA AACATCAATAACAGAAAGTGGGATGATAATAAGTTATAAAAT 2 t 13 | TLminic31 |
| 483 | 44 | 108 | ATATAA AACAAAAGAAAAATACAGAGAGTCAAAGAACATTGATGATAAT 3 t 3 | TLminic46 |
| 484 | 43 | 102 | ATATAA AACAAAAAAAGAATACAAAGAATCGAGAAACATTAATGGTATGTt 7 | TLminic3 |
| 519 | 48 | 1047 | GATATA TAAACAACAATAAATTAACAGAGCAGAAAAATGGGAAAAAGATGAAGTt 4 | TLminic25 |
| 555 | 48 | 528 | AGTAAC AAACAAAAACAGAAAATGGATGCGAGTAAAATAGATAATGGT 2 t 12 | TLminic11 |
| 587 | 45 | 20016 | ATATAT AAATCTAATAAATATAGAAAGCTGTGAGATAACAAATAAGAACTt 12 | TLmcontig9 |
| 605 | 47 | 63961 | GATTAT AACTGTAATACGGTGGTAAATAGATTTAGTAGATGTAGAGATTGATt 16 | TLminic55 |
| 632 | 47 | 7771 | ATGTAA AATCGTATTAATAAGAATAAGCAGCTGTGGTATAGTGGTGAAT 2 t 24 | TLmcontig14 |
| 633 | 46 | 18466 | ATGTGT AAAATCATATTAATAGAGATGAATGACTGTGATATAGTGGTGAAAT 2 t 5 | TLmcontig12 |
| 669 | 47 | 7143 | ATATTA ACAAAAATAACAAAAAATTCATTGTCAGTTGTAGAGTTATATTAGAAt 9 | TLminic14 |
| 700 | 44 | 1135 | ATATAA AATACACACAGAAAAAATTAACAGAGCGGTGAAAATGGTGAATt 9 | TLminic57 |
| 727 | 47 | 4269 | ATTAGA ACATAACACAGCGAGAGAACAATGTTAGAGATATATACAGAAAGT 2 t 7 | TLminic27 |
Positions of the gRNAs are indicated by complementarity to the fully/mature edited mRNA and are numbered starting from the start codon (numbered 1) at the 5′ end (column 1) of the mature edited mRNA. Length of the complementarities (column 2) and the transcript abundance (RPM; column 3) of the gRNAs were determined by removing the ‘extra’ 3′ U-tail to avoid the interruption. In the exact sequences (column 4), the 5′ non-complementary region (bold) with an RYAYA motif are underlined, and lowercase ‘t's with subscripts at the 3′ end indicate adenosines added post-transcriptionally. Minicircle names designating the sequence are shown beside each identifiable gRNA (column 5).
*RPM = Reads per million mapped reads.