Table 2.
Most common gRNA initiation sequences in T. lewisi
| Initiating sequence | Number of sequence classes | % | RPM | % |
|---|---|---|---|---|
| 5′ ATATAA | 31 | 18.3% | 93546 | 9.4% |
| 5′ ATATAT | 16 | 9.5% | 118150 | 11.8% |
| 5′ ATGTAA | 8 | 4.7% | 31416 | 3.1% |
| 5′ ATATTA | 7 | 4.1% | 25721 | 2.6% |
| 5′ GATATA | 5 | 3.0% | 37508 | 3.8% |
| 5′ ATAATA | 4 | 2.4% | 47033 | 4.7% |
| 5′ ATATTT | 4 | 2.4% | 13550 | 1.4% |
| 5′ ATGATA | 4 | 2.4% | 10647 | 1.1% |
| 5′ ATGTAT | 4 | 2.4% | 13860 | 1.4% |
| 5′ ATTAAA | 4 | 2.4% | 21115 | 2.1% |
| 5′ GTATAT | 4 | 2.4% | 15514 | 1.6% |
| 5′ ATATAG | 3 | 1.8% | 41447 | 4.1% |
| 5′ ATGTTA | 3 | 1.8% | 13229 | 1.3% |
| 5′ATTTAA | 3 | 1.8% | 37917 | 3.8% |
The major sequence classes identified were grouped based on the first 6 nt and sorted based on both the number of sequence classes and the total number of transcripts found. Most transcripts (25.3%) initiated with ATATA (includes ATATAA (9.4%), ATATAT (11.8%) and ATATAG (4.1%)).