Table 1.

List of primers used in transformation and sequencing (5′ – 3′)

pMTnCat vector primers
Pr_cat_F	ACTTTATTAATTCTAAATACTAGGGCCCCCCCTCGAGGTC
Pr_cat_R	ACTTTATTAATTCTAAATACTAGCGGCCGCTCTAGAACTA
p_Pr_F	TAGTATTTAGAATTAATAAAGTTTTTACACAATTATACGGACTTTATCAGCTA
p_Pr _R	TAGTATTTAGAATTAATAAAGTTTTTACACAATTATACGGACTTTATCTAGTC
Illumina sequencing primers (first PCR)
Tn-select-PA	TTTTACACAATTATACGG
R1-PA	ACACTCTTTCCCTACACGACGCTCTTC
PCR primers for enrichment (together universal Illumina primer), the nested mix was composed of an equimolar mixture of:
R2-Tn-select+1	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTVTTTTACACAATTATACGGAC
R2-Tn-select+2	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTVVTTTTACACAATTATACGGAC
R2-Tn-select+3	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTVVVTTTTACACAATTATACGGAC
R2-Tn-select+4	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTVVVVTTTTACACAATTATACGGAC