Table 2.

Previously published methods included in the comparative

Distribution	Reference	Metric	Priors	Estimate	Criteria
Poisson	Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium (2015).	Linear density	Goldset for E and NE	Probability fit	Absolute
Gamma	Defining the ABC of gene essentiality in streptococci (Amelia R. L. Charbonneau, 2017)	Linear density	Goldset for E+intergenic for NE	Probability fit	Fold change
Gumbel	Bayesian analysis of gene essentiality based on sequencing of transposon insertion libraries. Bioinformatics, 29 (6):695–703	Linear density	Goldset for E+intergenic for NE	Probability fit	Fold change
Hidden Markov Model	A Hidden Markov Model for identifying essential and growth-defect regions in bacterial genomes from transposon insertion sequencing data	Linear density and read values	Goldset	States	3