Table 1.
Single particle cryo-EM data collection, processing and model building for Escherichia coli RNAP-promoter open complex (RPo) with SspA
| Data collection and processing | |
| Microscope | Titan Krios |
| Voltage (kV) | 300 |
| Detector | K2 summit |
| Electron exposure (e/Å2) | 59 |
| Defocus range (μm) | 1.5–2.5 |
| Data collection mode | counting |
| Physical pixel size (Å/pixel) | 1.307 |
| Symmetry imposed | C1 |
| Initial particle images | 614,193 |
| Final particle images | 60,145 |
| Map resolution (Å)a | 3.68 |
| Refinement | |
| Map sharpening B-factor (Å) | –104 |
| Root-mean-square deviation | |
| Bond length (Å) | 0.007 |
| Bond angle (°) | 0.782 |
| Molprobity statistics | 2.93 |
| Clashscore | 11.70 |
| Rotamer outliers (%) | 10.60 |
| Cβ outliers (%) | 0.0 |
| Ramachandran plot | 99.88 |
| Favored (%) | 87.38 |
| Outliers (%) | 0.12 |
aGold-standard FSC 0.143 cutoff criteria.